ARSB Polyklonaler Antikörper

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• KD/KO Validated

ARSB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13227-1-AP

Synonyme

EC:3.1.6.12, N-acetylgalactosamine-4-sulfatase

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  WB analysis of HepG2 using 13227-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13227-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Mammakarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

13227-1-AP bindet in WB, IHC, IF, ELISA ARSB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARSB fusion protein Ag4083
• Vollständiger Name: arylsulfatase B
• Berechnetes Molekulargewicht: 413 aa, 46 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 44 kDa
• GenBank-Zugangsnummer: BC029051
• Gene symbol: ARSB
• Gene ID (NCBI): 411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Oxid Med Cell Longev; miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling.; Authors - Que Wang ; KD Validated
mouse	WB	Elife; Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.; Authors - Matthew R Blake
mouse	WB,IF	Biochem Biophys Rep; Generation of a novel disease model mouse for mucopolysaccharidosis type VI via c. 252T>C human ARSB mutation knock-in; Authors - Kosuke Hosoba