• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ASAH1 Monoklonaler Antikörper

ASAH1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B3C7

Kat-Nr. : 67092-2-PBS

Synonyme

ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase, AC

Filter:
  • Western Blot (WB) analysis of various lysates using ASAH1 Monoclonal antibody (67092-2-Ig)

    WB analysis using 67092-2-Ig (same clone as 67092-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67092-2-Ig (ASAH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASAH1 Monoclonal antibody (67092-2-Ig)

    IF Staining of MCF-7 using 67092-2-Ig (same clone as 67092-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ASAH1 antibody (67092-2-Ig, Clone: 2B3C7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67092-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67092-2-PBS bindet in WB, IF/ICC, Indirect ELISA ASAH1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ASAH1 fusion protein Ag28645
Vollständiger Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht 44-55 kDa
GenBank-ZugangsnummerBC016828
Gene symbol ASAH1
Gene ID (NCBI) 427
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.