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ASAP3 Polyklonaler Antikörper

ASAP3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14099-1-PBS

Synonyme

Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

Filter:
  • Western Blot (WB) analysis of various lysates using ASAP3 Polyclonal antibody (14099-1-AP)

    WB analysis using 14099-1-AP (same clone as 14099-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASAP3 Polyclonal antibody (14099-1-AP)

    IF Staining of HepG2 using 14099-1-AP (same clone as 14099-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASAP3 antibody (14099-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14099-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ASAP3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ASAP3 fusion protein Ag5231
Vollständiger Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Berechnetes Molekulargewicht 903 aa, 99 kDa
Beobachtetes Molekulargewicht100 kDa
GenBank-ZugangsnummerBC060786
Gene symbol ASAP3
Gene ID (NCBI) 55616
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.