SLC10A2/ASBT Polyklonaler Antikörper

SLC10A2/ASBT Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 25245-1-AP

Synonyme

ASBT, SLC10A2, Apical sodium-dependent bile acid transporter, Na(+)-dependent ileal bile acid transporter, ISBT

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• 

  WB analysis of mouse small intestine using 25245-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25245-1-AP (ASBT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 25245-1-AP

  IP result of anti-ASBT (IP:25245-1-AP, 4ug; Detection:25245-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe
• Erfolgreiche IP: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

25245-1-AP bindet in WB, IHC, IF, IP, ELISA SLC10A2/ASBT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC10A2/ASBT fusion protein Ag18808
• Vollständiger Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 2
• Berechnetes Molekulargewicht: 348 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC130523
• Gene symbol: ASBT
• Gene ID (NCBI): 6555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC10A2 also known as ASBT, ISBT, and NTCP2, is a sodium/bile acid cotransporter that is primarily responsible for the uptake of bile acids by apical cells in the distal ileum(PMID: 8661017). Mutations in SLC10A2 cause primary bile acid malabsorption (PBAM) and may be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG)(PMID: 9109432).

Publikationen

Species	Application	Title
mouse	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
human,mouse	WB,IHC	Cell Host Microbe; Veillonella intestinal colonization promotes C. difficile infection in Crohn's disease; Authors - Ziyu Yang
mouse	WB	Nat Commun; Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice; Authors - Xiaoxue Jiang
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

It does not work as expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver, mouse small intestine, mouse kidney, HepG2

SLC10A2/ASBT Antibody Western Blot validation (1:500 dilution) in Mouse liver, mouse small intestine, mouse kidney, HepG2 (Cat no:25245-1-AP)