ASBT Polyklonaler Antikörper

ASBT Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 25245-1-AP

Synonyme

ASBT, IBAT, ISBT, NTCP2, SLC10A2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse small intestine using 25245-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25245-1-AP (ASBT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 25245-1-AP

  IP Result of anti-ASBT (IP:25245-1-AP, 4ug; Detection:25245-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe
• Erfolgreiche IP: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below

Produktinformation

25245-1-AP bindet in WB, IP, ELISA ASBT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASBT fusion protein Ag18808
• Vollständiger Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 2
• Berechnetes Molekulargewicht: 348 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC130523
• Gene symbol: SLC10A2
• Gene ID (NCBI): 6555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu
mouse	WB	Food Funct; Apple polyphenol extract modulates bile acid metabolism and gut microbiota by regulating the circadian rhythms in daytime-restricted high fat diet feeding C57BL/6 male mice.; Authors - Yuan Cui
mouse	WB	Biochim Biophys Acta; Rhizoma Coptidis alkaloids alleviate hyperlipidemia in B6 mice by modulating gut microbiota and bile acid pathways.; Authors - Kai He
	WB	Biochim Biophys Acta Biomembr; S-acylation status of bile acid transporter hASBT regulates its function, metabolic stability, membrane expression, and phosphorylation state.; Authors - Ebehiremen N Ayewoh

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

It does not work as expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver, mouse small intestine, mouse kidney, HepG2

ASBT Antibody Western Blot validation (1:500 dilution) in Mouse liver, mouse small intestine, mouse kidney, HepG2 (Cat no:25245-1-AP)