ASC/TMS1 Monoklonaler Antikörper

ASC/TMS1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G12E6

Kat-Nr. : 67494-1-PBS

Synonyme

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

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  WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67494-1-Ig (same clone as 67494-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 67494-1-Ig (same clone as 67494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67494-1-PBS bindet in WB, IHC, IF/ICC, ELISA ASC/TMS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASC/TMS1 fusion protein Ag28424
• Vollständiger Name: PYD and CARD domain containing
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC004470
• Gene symbol: ASC/TMS1
• Gene ID (NCBI): 29108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.