ASCC2 Rekombinanter Antikörper

ASCC2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250566D5

Kat-Nr. : 86086-1-PBS

Synonyme

ASC-1 complex subunit p100, ASC1P100, RQT3

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  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86086-1-RR against Human ASCC2 were performed. The affinity constant is 26.5 pM.

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Geprüfte Anwendungen

Produktinformation

86086-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ASCC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ASCC2 fusion protein Ag2100
• Vollständiger Name: activating signal cointegrator 1 complex subunit 2
• Berechnetes Molekulargewicht: 757 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC025368
• Gene symbol: ASCC2
• Gene ID (NCBI): 84164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).