ASCC3 Rekombinanter Antikörper

ASCC3 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242755B4

Kat-Nr. : 85130-2-PBS

Synonyme

242755B4, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, ASC1p200, EC:3.6.4.12

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  WB analysis using 85130-2-RR (same clone as 85130-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85130-2-RR (ASCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85130-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01828-3

  Sandwich ELISA standard curve of MP01828-3, Human ASCC3 Recombinant Matched Antibody Pair - PBS only. 85130-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11848. 85130-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

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  Sample test of MP01828-3

  The mean ASCC3 concentration was determined to be 17.15 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 23.98 ng/mL in HepG2 cell extract based on a 1.80 mg/mL extract load.

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  IF Staining of HeLa using 85130-2-RR (same clone as 85130-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASCC3 antibody (85130-2-RR, Clone: 242755B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85130-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85130-2-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test ASCC3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ASCC3 fusion protein Ag11848
• Vollständiger Name: activating signal cointegrator 1 complex subunit 3
• Berechnetes Molekulargewicht: 111 aa, 13 kDa, 251 kDa
• Beobachtetes Molekulargewicht: 245 kDa
• GenBank-Zugangsnummer: BC050681
• Gene symbol: ASCC3
• Gene ID (NCBI): 10973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.