ASCL1 Polyklonaler Antikörper

ASCL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31206-1-AP

Synonyme

Achaete-scute homolog 1, ASH1, ASH-1, bHLHa46, Class A basic helix-loop-helix protein 46

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  WB analysis using 31206-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31206-1-AP (ASCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 31206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31206-1-AP (ASCL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31206-1-AP (ASCL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, NCI-H1299-Zellen, PC-3-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31206-1-AP bindet in WB, IHC, ELISA ASCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASCL1 fusion protein Ag35185
• Vollständiger Name: achaete-scute complex homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC003134
• Gene symbol: ASCL1
• Gene ID (NCBI): 429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa.