ASF1A Monoklonaler Antikörper

ASF1A Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D4A6

Kat-Nr. : 66884-1-PBS

Synonyme

2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98

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  WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66884-1-Ig (same clone as 66884-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1A antibody (66884-1-Ig, Clone: 2D4A6 ) at dilution of 1:500 and sms3AF647-1, CL488-Phalloidin (green). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51470-1

  Cytometric bead array sample test of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS.

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  Cytometric bead array standard curve of MP51470-1

  Cytometric bead array standard curve of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS. Standard:Ag1233. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66884-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA ASF1A und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASF1A fusion protein Ag1233
• Vollständiger Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 204 aa, 23 kDa
• Beobachtetes Molekulargewicht: 21-23 kDa
• GenBank-Zugangsnummer: BC010878
• Gene symbol: ASF1A
• Gene ID (NCBI): 25842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.