ASF1B-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ASF1B-specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 22258-1-AP

Synonyme

ASF1A/B, ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II

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  WB analysis using 22258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22258-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 22258-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 22258-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 22258-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22258-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF1B-specific antibody (22258-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

22258-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA ASF1B-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASF1B-specific fusion protein Ag17763
• Vollständiger Name: ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC010014
• Gene symbol: ASF1B
• Gene ID (NCBI): 55723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.

Publikationen

Species	Application	Title
human	WB	Nat Commun; RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.; Authors - Sumin Feng ; KD Validated
mouse	WB,IF	Epigenetics Chromatin; Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.; Authors - Xuemei Wang
human	ChIP	Cancer Lett; Activation of the FOXM1/ASF1B/PRDX3 axis confers hyperproliferative and antioxidative stress reactivity to gastric cancer; Authors - Zhou Zhao
mouse	WB	Int J Biol Macromol; Tannic acid protects against colitis by regulating the IL17 - NFκB and microbiota - methylation pathways; Authors - Minghui Wang
human	IHC	Tissue Cell; ASF1B is an essential prognostic indicator linked to the growth and resistance characteristics of bladder cancer; Authors - Peng Zhang
human	WB	bioRxiv; Mechanism of ASF1 Inhibition by CDAN1; Authors - Samantha F Sedor