ASF1B Rekombinanter Antikörper

ASF1B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250195A7

Kat-Nr. : 85848-1-RR

Synonyme

ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II

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  WB analysis using 85848-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85848-1-RR (ASF1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85848-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1B antibody (85848-1-RR, Clone: 250195A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85848-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85848-1-RR against Human ASF1B were performed. The affinity constant is 57.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HCT 116-Zellen, HEK-293-Zellen, Jurkat-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85848-1-RR bindet in WB, IF/ICC, ELISA ASF1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ASF1B fusion protein Ag17763
• Vollständiger Name: ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC010014
• Gene symbol: ASF1B
• Gene ID (NCBI): 55723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.