ASGR2 Polyklonaler Antikörper

ASGR2 Polyklonal Antikörper für WB, IHC, FC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11501-2-PBS

Synonyme

CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2

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  WB analysis of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 11501-2-AP (same clone as 11501-2-PBS)

  IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11501-2-AP (same clone as 11501-2-PBS)

  1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11501-2-PBS bindet in WB, IHC, FC, IP, Indirect ELISA ASGR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASGR2 fusion protein Ag2037
• Vollständiger Name: asialoglycoprotein receptor 2
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC017251
• Gene symbol: ASGR2
• Gene ID (NCBI): 433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.