ASH2L Polyklonaler Antikörper

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• KD/KO Validated

ASH2L Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12331-1-AP

Synonyme

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

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Galerie der Validierungsdaten

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  WB analysis of COS-7 using 12331-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12331-1-AP

  IP Result of anti-ASH2L (IP:12331-1-AP, 4ug; Detection:12331-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human gliomas using 12331-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12331-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, COLO 320-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

12331-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA ASH2L und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASH2L fusion protein Ag3008
• Vollständiger Name: ash2 (absent, small, or homeotic)-like (Drosophila)
• Berechnetes Molekulargewicht: 628 aa, 69 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC015936
• Gene symbol: ASH2L
• Gene ID (NCBI): 9070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.; Authors - Esteban A Orellana
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB,IHC,IF,IP,ChIP	Cancer Sci; ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.; Authors - Kai Zeng ; KD Validated
mouse	WB,IP	Mol Neurobiol; WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.; Authors - Minhan Ka