ASH2L Monoklonaler Antikörper

ASH2L Monoklonal Antikörper für Indirect ELISA, IHC, WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

Indirect ELISA, IHC, WB

Konjugation

Unkonjugiert

CloneNo.

1G1H3

Kat-Nr. : 67859-1-PBS

Synonyme

Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein

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  WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  WB analysis using 67859-1-Ig (same clone as 67859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67859-1-Ig (same clone as 67859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67859-1-Ig (same clone as 67859-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67859-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67859-1-PBS bindet in Indirect ELISA, IHC, WB ASH2L und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASH2L fusion protein Ag3008
• Vollständiger Name: ash2 (absent, small, or homeotic)-like (Drosophila)
• Berechnetes Molekulargewicht: 628 aa, 69 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC015936
• Gene symbol: ASH2L
• Gene ID (NCBI): 9070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.