ASIC1 Polyklonaler Antikörper

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• KD/KO Validated

ASIC1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 27235-1-AP

Synonyme

ACCN2, ASIC, Amiloride-sensitive cation channel 2, neuronal, Acid-sensing ion channel 1

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  WB analysis using 27235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 27235-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27235-1-AP

  IP result of anti-ASIC1 (IP:27235-1-AP, 4ug; Detection:27235-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

27235-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA ASIC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASIC1 fusion protein Ag25941
• Vollständiger Name: amiloride-sensitive cation channel 2, neuronal
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa, 80-90 kDa
• GenBank-Zugangsnummer: NM_001095
• Gene symbol: ASIC1
• Gene ID (NCBI): 41
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).

Publikationen

Species	Application	Title
mouse	WB,IF	Research (Wash D C); Targeting ASIC1a Promotes Neural Progenitor Cell Migration and Neurogenesis in Ischemic Stroke; Authors - Hongfei Ge
human,mouse	WB,CoIP	Int Immunopharmacol; ASIC1a regulates airway epithelial cell pyroptosis in acute lung injury by NLRP3-Caspase1-GSDMD pathway; Authors - Yuan-Yuan Tan
rat	WB	Cell Signal; Paeoniflorin inhibits pyroptosis of nucleus pulposus cells in an acidic environment and alleviates the degeneration of the intervertebral disc in rats; Authors - Wang-Ying Dai
human	IHC,FC	Lab Invest; Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a.; Authors - Xuewen Qian
mouse	FC	Lab Invest; ASIC1a promotes the proliferation of synovial fibroblasts via the ERK/MAPK pathway.; Authors - Jingjing Tao
mouse	WB	Eur J Pharmacol; The interaction of ASIC1a and ERS mediates nerve cell apoptosis induced by insulin deficiency.; Authors - Xuesheng Pan ; KD Validated