ASNA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ASNA1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15450-1-AP

Synonyme

ARSA, ARSA I, ARSA1, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15450-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15450-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15450-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15450-1-AP

  IP Result of anti-ASNA1 (IP:15450-1-AP, 4ug; Detection:15450-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 15450-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15450-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15450-1-AP

  Immunofluorescent analysis of HepG2 cells, using ASNA1 antibody 15450-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15450-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ASNA1 antibody (15450-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, humanes Herzgewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below

Produktinformation

15450-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ASNA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASNA1 fusion protein Ag7713
• Vollständiger Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Berechnetes Molekulargewicht: 38.7 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC002651
• Gene symbol: ASNA1
• Gene ID (NCBI): 439
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to DDP and arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.; Authors - Yasunori Yamamoto
human	WB	J Cell Sci; Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.; Authors - Janine Pfaff ; KD Validated
human	WB	J Cell Sci; An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.; Authors - Tarik Exner ; KD Validated
human	WB	J Cell Sci; A trap mutant reveals the physiological client spectrum of TRC40.; Authors - Javier Coy-Vergara ; KD Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata
human	WB	FEBS Lett; Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals.; Authors - Jing Yang