ASNS Monoklonaler Antikörper

ASNS Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C3D3

Kat-Nr. : 68004-1-Ig

Synonyme

1C3D3, Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11

---

• 

  WB analysis of K-562 using 68004-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68004-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68004-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68004-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68004-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 68004-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat pancreas using 68004-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 68004-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 68004-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASNS antibody (68004-1-Ig, Clone: 1C3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, 4T1-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68004-1-Ig bindet in WB, IHC, IF/ICC, ELISA ASNS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASNS fusion protein Ag6817
• Vollständiger Name: asparagine synthetase
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC014621
• Gene symbol: ASNS
• Gene ID (NCBI): 440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.