ASPN Polyklonaler Antikörper

ASPN Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27602-1-PBS

Synonyme

SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1

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  WB analysis of HepG2 using 27602-1-AP (same clone as 27602-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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  WB analysis using 27602-1-AP (same clone as 27602-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 27602-1-AP (same clone as 27602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27602-1-AP (ASPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27602-1-PBS bindet in WB, IHC, Indirect ELISA ASPN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASPN fusion protein Ag26380
• Vollständiger Name: asporin
• Berechnetes Molekulargewicht: 384 aa, 44 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC063114
• Gene symbol: ASPN
• Gene ID (NCBI): 54829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASPN, also known as periodontal ligament-associated protein1 (PLAP1), is an extracellular matrix (ECM) protein. The calculated molecular weight of ASPN is 43 kDa. It belongs to the small leucine-rich proteoglycans (SLRPs) family and contains a unique aspartate-rich N terminus that distinguishes it from other SLRPs family members. It was firstly identified in human cartilage and it was associated with the pathogenesis of bone and joint diseases, including osteoarthritis, intervertebral disc degeneration and periodontal ligament mineralization (PMID:11152692; 26089687).