ASS1 Monoklonaler Antikörper

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• KD/KO Validated

ASS1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1A11

Kat-Nr. : 66036-1-PBS

Synonyme

1D1A11, ASS, EC:6.3.4.5

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  WB analysis of pig liver using 66036-1-Ig (same clone as 66036-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66036-1-Ig (same clone as 66036-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66036-1-Ig (same clone as 66036-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66036-1-Ig (same clone as 66036-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66036-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ASS1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASS1 fusion protein Ag9314
• Vollständiger Name: argininosuccinate synthetase 1
• Berechnetes Molekulargewicht: 412 aa, 47 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC009243
• Gene symbol: ASS1
• Gene ID (NCBI): 445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).