ASXL1 Rekombinanter Antikörper

ASXL1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241563A5

Kat-Nr. : 84630-1-RR

Synonyme

241563A5, Additional sex combs-like protein 1, KIAA0978, Polycomb group protein ASXL1

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  WB analysis using 84630-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84630-1-RR (ASXL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84630-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84630-1-RR against Human ASXL1 were performed. The affinity constant is 39.3 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HL-60-Zellen, K-562-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84630-1-RR bindet in WB, ELISA ASXL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ASXL1 fusion protein Ag10290
• Vollständiger Name: additional sex combs like 1 (Drosophila)
• Berechnetes Molekulargewicht: 84aa,10 kDa; 1541aa,165 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: BC064984
• Gene symbol: ASXL1
• Gene ID (NCBI): 171023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Additional sex combs-like 1 (ASXL1) is a member of the ASXL family, which is involved in epigenetic regulation. It is one of the mammalian Asx homologs. Mutations in the ASXL1 gene are frequently found in myeloid neoplasms, breast cancers, and prostate cancers (PMID: 30013160; PMID: 22436456; PMID: 26470845). In patients with myeloid malignancies, ASXL1 mutations are mostly heterozygous frameshift or nonsense mutations that result in C-terminal truncation (PMID: 30982744; PMID: 26095772; PMID: 32683582; PMID: 29643185).