ASZ1 Polyklonaler Antikörper

ASZ1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21550-1-AP

Synonyme

ALP1, ANKL1, Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1, Ankyrin-like protein 1, ASZ 1

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  WB analysis of mouse testis using 21550-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21550-1-AP (ASZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21550-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21550-1-AP (ASZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21550-1-AP

  IP result of anti-ASZ1 (IP:21550-1-AP, 4ug; Detection:21550-1-AP 1:600) with mouse testis tissue lysate 1560 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

21550-1-AP bindet in WB, IF, IP, ELISA ASZ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASZ1 fusion protein Ag16217
• Vollständiger Name: ankyrin repeat, SAM and basic leucine zipper domain containing 1
• Berechnetes Molekulargewicht: 475 aa, 53 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC034963
• Gene symbol: ASZ1
• Gene ID (NCBI): 136991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF, WB	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding
mouse	IF	Dev Cell; TDRD1 phase separation drives intermitochondrial cement assembly to promote piRNA biogenesis and fertility; Authors - Jie Gao
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang
mouse	WB	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu
mouse	IF	Nucleic Acids Res; Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.; Authors - Deqiang Ding