ATAD2 Polyklonaler Antikörper

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• KD/KO Validated

ATAD2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 23894-1-AP

Synonyme

ANCCA, ATAD2, PRO2000

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23894-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23894-1-AP (ATAD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 23894-1-AP

  IP Result of anti-ATAD2 (IP:23894-1-AP, 4ug; Detection:23894-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 23894-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23894-1-AP (ATAD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

23894-1-AP bindet in WB, IP, IF, ELISA ATAD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATAD2 fusion protein Ag20947
• Vollständiger Name: ATPase family, AAA domain containing 2
• Berechnetes Molekulargewicht: 1390 aa, 159 kDa
• Beobachtetes Molekulargewicht: 159 kDa
• GenBank-Zugangsnummer: BC113656
• Gene symbol: ATAD2
• Gene ID (NCBI): 29028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATAD2 is one of them encoding a conserved factor harbouring an AAA type ATPase domain and a bromodomain. ATAD2 controls chromatin dynamics, genome transcriptional activities and apoptotic cell response. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
		Cell Rep; Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication; Authors - Vanesa Alvarez
human	WB	Biomed Pharmacother; Silencing METTL3 inhibits the proliferation and invasion of osteosarcoma by regulating ATAD2.; Authors - Lei Zhou
human	WB,IF	Mol Cell Biol; Mutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.; Authors - Pavlo Lazarchuk ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; ATAD2 drives colorectal cancer progression by regulating TRIM25 expression via a positive feedback loop with E2F transcriptional factors.; Authors - Yalin Tong ; KD Validated
human	WB	iScience; Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2; Authors - Shalabh Shukla