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ATAD3A/B Rekombinanter Antikörper

ATAD3A/B Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251342C9

Kat-Nr. : 86461-2-PBS

Synonyme

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

Filter:
  • Western Blot (WB) analysis of various lysates using ATAD3A/B Recombinant antibody (86461-2-RR)

    WB analysis using 86461-2-RR (same clone as 86461-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEPG2 cells using ATAD3A/B Recombinant antibody (86461-2-RR)

    IF Staining of HEPG2 using 86461-2-RR (same clone as 86461-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86461-2-RR

    Affinity and Kinetic Characterization of 86461-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

Geprüfte Anwendungen

Produktinformation

86461-2-PBS bindet in WB, IF/ICC, Indirect ELISA ATAD3A/B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ATAD3A/B fusion protein Ag9836
Vollständiger Name ATPase family, AAA domain containing 3B
Berechnetes Molekulargewicht 72 kDa
Beobachtetes Molekulargewicht65-70 kDa
GenBank-ZugangsnummerBC002542
Gene symbol ATAD3B
Gene ID (NCBI) 83858
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.