ATAD3A/B Rekombinanter Antikörper

ATAD3A/B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251342C9

Kat-Nr. : 86461-2-RR

Synonyme

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

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  WB analysis using 86461-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEPG2 using 86461-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86461-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maushirngewebe, Mausnierengewebe, Rattennierengewebe, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86461-2-RR bindet in WB, IF/ICC, ELISA ATAD3A/B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATAD3A/B fusion protein Ag9836
• Vollständiger Name: ATPase family, AAA domain containing 3B
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC002542
• Gene symbol: ATAD3B
• Gene ID (NCBI): 83858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.