ATF1 Polyklonaler Antikörper

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ATF1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11946-1-PBS

Synonyme

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

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  WB analysis of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11946-1-AP (same clone as 11946-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11946-1-AP (same clone as 11946-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11946-1-AP (same clone as 11946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (11946-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11946-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11946-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA ATF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF1 fusion protein Ag2548
• Vollständiger Name: activating transcription factor 1
• Berechnetes Molekulargewicht: 271 aa, 29 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC029619
• Gene symbol: ATF1
• Gene ID (NCBI): 466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).