ATF2 Polyklonaler Antikörper

ATF2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11908-1-PBS

Synonyme

CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2

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  WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

  IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11908-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA ATF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF2 fusion protein Ag2581
• Vollständiger Name: activating transcription factor 2
• Berechnetes Molekulargewicht: 209 aa, 23 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 64 kDa
• GenBank-Zugangsnummer: BC026175
• Gene symbol: ATF2
• Gene ID (NCBI): 1386
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).