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ATF4 Polyklonaler Antikörper
ATF4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Ratte und mehr (3)
Anwendung
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10835-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, HEK-293-Zellen, Rattenhirngewebe |
| Erfolgreiche IP | HEK-293-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | mit Tunicamycin behandelte HeLa-Zellen, HeLa-Zellen, HepG2-Zellen |
| Erfolgreiche Detektion in FC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
Produktinformation
10835-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA ATF4 und zeigt Reaktivität mit human, Ratten
| Getestete Reaktivität | human, Ratte |
| In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Ratte, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | ATF4 fusion protein Ag1279 |
| Vollständiger Name | activating transcription factor 4 (tax-responsive enhancer element B67) |
| Berechnetes Molekulargewicht | 39 kDa |
| Beobachtetes Molekulargewicht | 45-50 kDa |
| GenBank-Zugangsnummer | BC022088 |
| Gene symbol | ATF4 |
| Gene ID (NCBI) | 468 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
What is the molecular weight of ATF4?
The molecular weight of ATF is 38.6 kD.
What is ATF4?
Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).
What the function of ATF4?
ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).
What is the effect of ATF4 interaction with RSK2?
ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).
Where is ATF4 expressed?
ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).
What regulates ATF4 expression?
ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).
How does ATF4 expression affect Ocn mRNA?
Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).
Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?
Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for ATF4 antibody 10835-1-AP | Protokoll herunterladen |
| IHC protocol for ATF4 antibody 10835-1-AP | Protokoll herunterladen |
| IF protocol for ATF4 antibody 10835-1-AP | Protokoll herunterladen |
| IP protocol for ATF4 antibody 10835-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. | ||
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Nucleic Acids Res Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xu-Qiao (Verified Customer) (10-24-2023) | Although there is a band at 50 kd, many other non-specific bands.
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FH Lucile (Verified Customer) (08-01-2023) | The ATF4 antibody worked well for immunoprecipitation, western blotting (1:1000 but based on initial results could dilute further) and gave a very bright IF signal on fixed smooth muscle cells.
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FH SU (Verified Customer) (03-22-2022) |
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FH Natalie (Verified Customer) (12-11-2018) | Antibody worked well, although does detect some non-specific bands which appear to be dimers from the molecular weight.
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