ATF4 Polyklonaler Antikörper

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• KD/KO Validated

ATF4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10835-1-PBS

Synonyme

cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, TXREB

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  WB analysis of A431 using 10835-1-AP (same clone as 10835-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 10835-1-AP (same clone as 10835-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

  IP result of anti-ATF4 (IP:10835-1-AP, 3ug; Detection:10835-1-AP 1:400) with HEK-293 cells lysate 6000ug. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10835-1-AP (same clone as 10835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 10835-1-AP (same clone as 10835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 10835-1-AP (same clone as 10835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10835-1-AP (same clone as 10835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10835-1-AP (same clone as 10835-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (10835-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATF4 (10835-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10835-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA ATF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF4 fusion protein Ag1279
• Vollständiger Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC022088
• Gene symbol: ATF4
• Gene ID (NCBI): 468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)