ATF4 Rekombinanter Antikörper

ATF4 Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

5N3

Kat-Nr. : 81798-1-PBS

Synonyme

5N3, cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67

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  WB analysis of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

  Non-treated HEK-293 and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 81798-1-RR (same clone as 81798-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81798-1-RR (same clone as 81798-1-PBS)

  Non-treated HeLa and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81798-1-RR (ATF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 81798-1-RR (same clone as 81798-1-PBS)

  IP result of anti-ATF4 (IP:81798-1-RR, 4ug; Detection:81798-1-RR 1:4000) with HEK-293 cells lysate 1440 ug. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81798-1-RR (same clone as 81798-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81798-1-RR (ATF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  ChIP experiment of NIH/3T3 using 81798-1-RR (same clone as 81798-1-PBS)

  Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/mll) overnight. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of ATF4 (81798-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81798-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81798-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81798-1-RR against Human ATF4 were performed. The affinity constant is 0.500 nM.

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Geprüfte Anwendungen

Produktinformation

81798-1-PBS bindet in WB, IHC, IP, Indirect ELISA, ChIP-qPCR ATF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATF4 fusion protein Ag1279
• Vollständiger Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC022088
• Gene symbol: ATF4
• Gene ID (NCBI): 468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.