ATF4 Rekombinanter Antikörper

ATF4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

230306B5

Kat-Nr. : 81798-2-PBS

Synonyme

230306B5, cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67

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  WB analysis using 81798-2-RR (same clone as 81798-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81798-2-RR (ATF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81798-2-RR (same clone as 81798-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (81798-2-RR, Clone: 230306B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81798-2-RR (same clone as 81798-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug ATF4 Recombinant antibody (81798-2-RR, Clone:230306B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00113-1

  Cytometric bead array standard curve of MP00113-1, ATF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81798-2-PBS. Detection antibody: 81798-5-PBS. Standard: Ag1279. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00113-3

  Cytometric bead array standard curve of MP00113-3, ATF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81798-2-PBS. Detection antibody: 81798-4-PBS. Standard: Ag1279. Range: 1.25-20 ng/mL.

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  ChIP experiment of NIH/3T3 using 81798-2-RR (same clone as 81798-2-PBS)

  Chromatin was prepared from NIH/3T3 cells treated with Tunicamycin (2ug/mll) overnight. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of ATF4 (81798-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81798-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81798-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81798-2-RR against Human ATF4 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

81798-2-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, ChIP-qPCR ATF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATF4 fusion protein Ag1279
• Vollständiger Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC022088
• Gene symbol: ATF4
• Gene ID (NCBI): 468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer.