• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATF5 Monoklonaler Antikörper

ATF5 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E5B6

Kat-Nr. : 67066-1-PBS

Synonyme

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx

Filter:
  • Western Blot (WB) analysis of various lysates using ATF5 Monoclonal antibody (67066-1-Ig)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATF5 Monoclonal antibody (67066-1-Ig)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67066-1-PBS bindet in WB, Indirect ELISA ATF5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ATF5 fusion protein Ag7214
Vollständiger Name activating transcription factor 5
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 30-35 kDa
GenBank-ZugangsnummerBC005174
Gene symbol ATF5
Gene ID (NCBI) 22809
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).