ATF6 Monoklonaler Antikörper

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ATF6 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7E4

Kat-Nr. : 66563-1-PBS

Synonyme

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

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  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66563-1-Ig (same clone as 66563-1-PBS)

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66563-1-Ig (same clone as 66563-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66563-1-Ig (same clone as 66563-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66563-1-Ig (same clone as 66563-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66563-1-Ig (same clone as 66563-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF6 antibody (66563-1-Ig, Clone: 3B7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (66563-1-Ig, Clone:3B7E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66563-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA ATF6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATF6 fusion protein Ag21456
• Vollständiger Name: activating transcription factor 6
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC014969
• Gene symbol: ATF6
• Gene ID (NCBI): 22926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.