ATF6 Rekombinanter Antikörper

ATF6 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2O8

Kat-Nr. : 82278-1-PBS

Synonyme

2O8, activating transcription factor 6, ATF 6, ATF6 alpha, ATF6A

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  WB analysis using 82278-1-RR (same clone as 82278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82278-1-RR (ATF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

  WB result of ATF6 antibody (82278-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

  IP result of anti-ATF6 (IP:82278-1-RR, 4ug; Detection:82278-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82278-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82278-1-RR against Human ATF6 were performed. The affinity constant is 0.344 nM.

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Geprüfte Anwendungen

Produktinformation

82278-1-PBS bindet in WB, IP, Indirect ELISA ATF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATF6 fusion protein Ag21456
• Vollständiger Name: activating transcription factor 6
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC014969
• Gene symbol: ATF6
• Gene ID (NCBI): 22926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).