ATF7IP Polyklonaler Antikörper

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• KD/KO Validated

ATF7IP Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14699-1-AP

Synonyme

ATF-interacting protein, ATFa-associated modulator, ATF7-interacting protein, ATF7, ATF

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  IHC staining of human breast cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

14699-1-AP bindet in WB, IHC, IF, ELISA ATF7IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF7IP fusion protein Ag6341
• Vollständiger Name: activating transcription factor 7 interacting protein
• Berechnetes Molekulargewicht: 137 kDa
• GenBank-Zugangsnummer: BC063855
• Gene symbol: ATF7IP
• Gene ID (NCBI): 55729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF7IP (Activating Transcription Factor 7 Interacting Protein), also known as MCAF1 (MBD1-containing chromatin-associated factor 1), is a multifunctional scaffold protein that plays a pivotal role in epigenetic regulation and transcriptional control. Functionally, ATF7IP acts as an adaptor that recruits the histone methyltransferase SETDB1 to specific genomic loci, facilitating the deposition of the heterochromatin mark H3K9me3 and silencing target genes, including retrotransposons such as LINE1. This activity is essential for maintaining genomic stability by suppressing transposable element mobilization and preventing DNA damage.

Publikationen

Species	Application	Title
human	WB,IF	Hum Mol Genet; Mechanism suppressing H3K9 trimethylation in pluripotent stem cells and its demise by polyQ-expanded huntingtin mutations.; Authors - Dilber Irmak ; KD Validated