ATG12 Polyklonaler Antikörper

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• KD/KO Validated

ATG12 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11264-1-PBS

Synonyme

FBR93, Autophagy-related protein 12, APG12-like, APG12L, APG12 like

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  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 11264-1-AP (same clone as 11264-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11264-1-AP (same clone as 11264-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATG12 antibody (11264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11264-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATG12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG12 fusion protein Ag1791
• Vollständiger Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC012266
• Gene symbol: ATG12
• Gene ID (NCBI): 9140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.