ATG12 Rekombinanter Antikörper

ATG12 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242483F1

Kat-Nr. : 84935-6-PBS

Synonyme

242483F1, APG12, APG12 like, APG12L, APG12-like

---

• 

  WB analysis using 84935-6-RR (same clone as 84935-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84935-6-RR (ATG12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84935-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84935-6-RR against Human ATG12 were performed. The affinity constant is 0.637 nM.

---

Geprüfte Anwendungen

Produktinformation

84935-6-PBS bindet in WB, IHC, Indirect ELISA ATG12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATG12 fusion protein Ag1791
• Vollständiger Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC012266
• Gene symbol: ATG12
• Gene ID (NCBI): 9140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.