ATG13 Rekombinanter Antikörper

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• KD/KO Validated

ATG13 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G14

Kat-Nr. : 82718-3-PBS

Synonyme

KIAA0652, 3G14, Autophagy related protein 13, Autophagy-related protein 13

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  WB analysis using 82718-3-RR (same clone as 82718-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 82718-3-RR (same clone as 82718-3-PBS)

  WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells. This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82718-3-RR (same clone as 82718-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82718-3-RR (ATG13 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82718-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82718-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82718-3-PBS bindet in WB, IHC, Indirect ELISA ATG13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATG13 fusion protein Ag13090
• Vollständiger Name: KIAA0652
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001331
• Gene symbol: ATG13
• Gene ID (NCBI): 9776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)