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ATG16L1 Polyklonaler Antikörper

ATG16L1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 29445-1-AP

Synonyme

APG16 like 1, APG16L, APG16-like 1, ATG16L, Autophagy-related protein 16-1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBCOLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

29445-1-AP bindet in WB, IHC, IF/ICC, ELISA ATG16L1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATG16L1 fusion protein Ag31318
Vollständiger Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Berechnetes Molekulargewicht 607 aa, 68 kDa
Beobachtetes Molekulargewicht68-75 kDa
GenBank-ZugangsnummerBC000061
Gene symbol ATG16L1
Gene ID (NCBI) 55054
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 66 kDa; and Atg16L1β, 68 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ATG16L1 antibody 29445-1-APProtokoll herunterladen
IHC protocol for ATG16L1 antibody 29445-1-APProtokoll herunterladenl
IF protocol for ATG16L1 antibody 29445-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Nanobiotechnology

Engineering exosomes derived from TNF-α preconditioned IPFP-MSCs enhance both yield and therapeutic efficacy for osteoarthritis

Authors - Jiangyi Wu
humanIHC

Cell Signal

Inhibition of XIST restrains paclitaxel resistance in breast cancer cells by targeting hsa-let-7d-5p/ATG16L1 through regulation of autophagy

Authors - Yueyue Wang
mouseWB

Food Funct

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Authors - Jian Fang
humanWB

Anticancer Drugs

Purvalanol A induces apoptosis and reverses cisplatin resistance in ovarian cancer

Authors - Xiaoyi Zhang
WB

Autophagy

Regulation of N-Degron Recognin-Mediated Autophagy by the sars-cov-2 plpro ubiquitin deconjugase

Authors - Carlos Ayala Torres
humanWB,IF

J Cell Mol Med

CTSL Promotes Autophagy in Laryngeal Cancer Through the IL6-JAK-STAT3 Signalling Pathway

Authors - Xueying Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boel (Verified Customer) (07-30-2025)

25µg gastrocnemius muscle total protein extract from mdx mouse (dystrophin deficient mice that are used as a model for Duchenne muscular dystrophy) generated a strong band of full lenght protein plus multiple additional bands of lower molecular weight. 25µg of total protein extract from pooled wild type zebrafish 5 days post fertilization generated no protein bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: mouse muscle, zebrafish total extract
ATG16L1 Antibody Western Blot validation (1/500 dilution) in mouse muscle, zebrafish total extract (Cat no:29445-1-AP)