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ATG16L2 Polyklonaler Antikörper

ATG16L2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24322-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse spleen tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    WB analysis of mouse spleen using 24322-1-AP (same clone as 24322-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    WB analysis of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    IP experiment of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

    IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ATG16L2 Polyclonal antibody (24322-1-AP)

    IF Staining of MCF-7 using 24322-1-AP (same clone as 24322-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24322-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA ATG16L2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATG16L2 fusion protein Ag19399
Vollständiger Name ATG16 autophagy related 16-like 2 (S. cerevisiae)
Berechnetes Molekulargewicht 619 aa, 69 kDa
Beobachtetes Molekulargewicht 70 kDa, 30 kDa
GenBank-ZugangsnummerBC137490
Gene symbol ATG16L2
Gene ID (NCBI) 89849
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.