ATG2A Polyklonaler Antikörper

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• KD/KO Validated

ATG2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23226-1-PBS

Synonyme

KIAA0404, Autophagy-related protein 2 homolog A

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  WB analysis using 23226-1-AP (same clone as 23226-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23226-1-AP (same clone as 23226-1-PBS)

  IP result of anti-ATG2A (IP:23226-1-AP, 4ug; Detection:23226-1-AP 1:4000) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 23226-1-AP (same clone as 23226-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATG2A antibody (23226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23226-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ATG2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG2A fusion protein Ag19709
• Vollständiger Name: ATG2 autophagy related 2 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 1938 aa, 213 kDa
• Beobachtetes Molekulargewicht: 215 kDa
• GenBank-Zugangsnummer: BC110650
• Gene symbol: ATG2A
• Gene ID (NCBI): 23130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.