ATG4B Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG4B Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5A7

Kat-Nr. : 67727-1-PBS

Synonyme

Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B

---

• 

  WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 67727-1-Ig (same clone as 67727-1-PBS)

  WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67727-1-Ig (same clone as 67727-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67727-1-PBS bindet in WB, IHC, IF/ICC, ELISA ATG4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATG4B fusion protein Ag30510
• Vollständiger Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000719
• Gene symbol: ATG4B
• Gene ID (NCBI): 23192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.