ATG4D Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG4D Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16924-1-AP

Synonyme

APG4 D, APG4D, ATG, AUTL4, AUT-like 4 cysteine endopeptidase

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16924-1-AP (ATG4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 16924-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG4D antibody (16924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below

Produktinformation

16924-1-AP bindet in WB, IF/ICC, ELISA ATG4D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG4D fusion protein Ag10307
• Vollständiger Name: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Berechnetes Molekulargewicht: 474 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC068992
• Gene symbol: ATG4D
• Gene ID (NCBI): 84971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma.; Authors - Tianzhi Huang
human	WB	Mol Cell; Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.; Authors - Joanne Durgan ; KO Validated
human,mouse	WB	ACS Sens; Air-Liquid Interface Microfluidic Monitoring Sensor Platform for Studying Autophagy Regulation after PM2.5 Exposure; Authors - Lulu Zheng
human	WB	Cell Rep; Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation; Authors - Rachel Ulferts ; KD Validated
human	WB	Mol Ther Nucleic Acids; lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.; Authors - Changhong Liu
human	WB	Autophagy; Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.; Authors - Alexander Agrotis ; KD Validated