ATG4D Rekombinanter Antikörper

ATG4D Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241035G8

Kat-Nr. : 83951-4-PBS

Synonyme

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

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  WB analysis of A549 using 83951-4-RR (same clone as 83951-4-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83951-4-RR (same clone as 83951-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83951-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83951-4-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ATG4D und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATG4D fusion protein Ag10177
• Vollständiger Name: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Berechnetes Molekulargewicht: 474 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC068992
• Gene symbol: ATG4D
• Gene ID (NCBI): 84971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.