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ATG5 Monoklonaler Antikörper
ATG5 Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2D3B8
Kat-Nr. : 66744-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunhistochemie (IHC) | IHC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 6 publications below |
WB | See 46 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Produktinformation
66744-1-Ig bindet in WB, IHC, IF, IP, ELISA ATG5 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | ATG5 fusion protein Ag16685 |
Vollständiger Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
Berechnetes Molekulargewicht | 32 kDa |
Beobachtetes Molekulargewicht | 50-55 and 40-45 kDa |
GenBank-Zugangsnummer | BC002699 |
Gene symbol | ATG5 |
Gene ID (NCBI) | 9474 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for ATG5 antibody 66744-1-Ig | Protokoll herunterladen |
IHC protocol for ATG5 antibody 66744-1-Ig | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy | ||
Dev Cell ATG5 attenuates inflammatory signaling in mouse embryonic stem cells to control differentiation
| ||
J Nanobiotechnology Cold exposure protects against medial arterial calcification development via autophagy | ||
J Nanobiotechnology Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.
| ||
Biochim Biophys Acta Mol Basis Dis Hydroxysafflor yellow A inhibits neuronal ferroptosis and ferritinophagy in ischemic stroke |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marina (Verified Customer) (11-22-2021) | ATG 5 and the complex ATG5-ATG12 are visible, almost no background.
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