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  • KD/KO Validated

ATG5 Monoklonaler Antikörper

ATG5 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

CloneNo.

2D3B8

Kat-Nr. : 66744-1-Ig

Synonyme

2D3B8, APG5, APG5 LIKE, APG5L, APG5-like



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66744-1-Ig bindet in WB, IHC, IF, IP, ELISA ATG5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ATG5 fusion protein Ag16685
Vollständiger Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 32 kDa
Beobachtetes Molekulargewicht 50-55 and 40-45 kDa
GenBank-ZugangsnummerBC002699
Gene symbol ATG5
Gene ID (NCBI) 9474
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ATG5 antibody 66744-1-IgProtokoll herunterladen
IHC protocol for ATG5 antibody 66744-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
humanWB

Adv Sci (Weinh)

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Authors - Lihao Jin
human,mouseWB,IP

Dev Cell

ATG5 attenuates inflammatory signaling in mouse embryonic stem cells to control differentiation

Authors - Sheng Li
  • KD Validated
humanWB

J Nanobiotechnology

Cold exposure protects against medial arterial calcification development via autophagy

Authors - Fu-Xing-Zi Li
humanWB

J Nanobiotechnology

Reciprocal regulation of NRF2 by autophagy and ubiquitin-proteasome modulates vascular endothelial injury induced by copper oxide nanoparticles.

Authors - Na Li
  • KO Validated
mouseWB

Biochim Biophys Acta Mol Basis Dis

Hydroxysafflor yellow A inhibits neuronal ferroptosis and ferritinophagy in ischemic stroke

Authors - Yige Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Marina (Verified Customer) (11-22-2021)

ATG 5 and the complex ATG5-ATG12 are visible, almost no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
  • Cell Tissue Type: primary mouse microglia