ATG5 Monoklonaler Antikörper

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• KD/KO Validated

ATG5 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D3B8

Kat-Nr. : 66744-1-PBS

Synonyme

2D3B8, APG5, APG5 LIKE, APG5L, APG5-like

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  WB analysis using 66744-1-Ig (same clone as 66744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66744-1-Ig (same clone as 66744-1-PBS)

  WB result of ATG5 antibody (66744-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66744-1-Ig (same clone as 66744-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66744-1-Ig (ATG5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66744-1-Ig (same clone as 66744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66744-1-Ig (ATG5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66744-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66744-1-PBS bindet in WB, IHC, Indirect ELISA ATG5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATG5 fusion protein Ag16685
• Vollständiger Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 50-55 and 40-45 kDa
• GenBank-Zugangsnummer: BC002699
• Gene symbol: ATG5
• Gene ID (NCBI): 9474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862)