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ATG7 Polyklonaler Antikörper

ATG7 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28413-1-PBS

Synonyme

GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L

Filter:
  • Western Blot (WB) analysis of various lysates using ATG7 Polyclonal antibody (28413-1-AP)

    WB analysis using 28413-1-AP (same clone as 28413-1-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28413-1-AP (ATG7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATG7 Polyclonal antibody (28413-1-AP)

    WB analysis of HeLa using 28413-1-AP (same clone as 28413-1-PBS)

    WB result of ATG7 antibody (28413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells. This data was developed using the same antibody clone with 28413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ATG7 Polyclonal antibody (28413-1-AP)

    IHC staining of human cervical cancer using 28413-1-AP (same clone as 28413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28413-1-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28413-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28413-1-PBS bindet in WB, IHC, Indirect ELISA ATG7 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATG7 fusion protein Ag29172
Vollständiger Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 78 kDa
Beobachtetes Molekulargewicht68-70 kDa
GenBank-ZugangsnummerBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.