ATG7 Monoklonaler Antikörper

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• KD/KO Validated

ATG7 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B5A10

Kat-Nr. : 67341-1-PBS

Synonyme

Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L, APG7 LIKE

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  WB analysis using 67341-1-Ig (same clone as 67341-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis using 67341-1-Ig (same clone as 67341-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67341-1-Ig (same clone as 67341-1-PBS)

  WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  WB result of ATG7 antibody (67341-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67341-1-Ig (same clone as 67341-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 67341-1-Ig (same clone as 67341-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67341-1-Ig (same clone as 67341-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67341-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ATG7 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATG7 fusion protein Ag29172
• Vollständiger Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 68-78 kDa
• GenBank-Zugangsnummer: BC000091
• Gene symbol: ATG7
• Gene ID (NCBI): 10533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.