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ATG7 Monoklonaler Antikörper

ATG7 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B5A10

Kat-Nr. : CL488-67341

Synonyme

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ATG7 Monoclonal anti (CL488-67341)

    IF Staining of HeLa using CL488-67341

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated ATG7 antibody (CL488-67341, Clone: 2B5A10 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ATG7 Monoclonal anti (CL488-67341)

    IF Staining of HepG2 using CL488-67341

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 ATG7 antibody (CL488-67341, Clone: 2B5A10 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67341 bindet in IF ATG7 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ATG7 fusion protein Ag29172
Vollständiger Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 78 kDa
Beobachtetes Molekulargewicht 68-78 kDa
GenBank-ZugangsnummerBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 ATG7 antibody CL488-67341Protokoll herunterladen
Standard-Protokolle
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