ATG7 Monoklonaler Antikörper

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• KD/KO Validated

ATG7 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2B5A10

Kat-Nr. : CL594-67341

Synonyme

Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L, APG7 LIKE

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  IF Staining of HepG2 using CL594-67341

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 ATG7 antibody (CL594-67341, Clone: 2B5A10 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-67341

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 ATG7 antibody (CL594-67341, Clone: 2B5A10 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-67341

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ATG7 (CL594-67341, Clone:2B5A10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67341 bindet in IF/ICC, FC (Intra) ATG7 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATG7 fusion protein Ag29172
• Vollständiger Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 68-78 kDa
• GenBank-Zugangsnummer: BC000091
• Gene symbol: ATG7
• Gene ID (NCBI): 10533
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.