GABARAPL1 Rekombinanter Antikörper

GABARAPL1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240533F5

Kat-Nr. : 83817-1-RR

Synonyme

240533F5, APG8 LIKE, APG8L, ATG8, ATG8L

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  WB analysis using 83817-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83817-1-RR (ATG8L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83817-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83817-1-RR (GABARAPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83817-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83817-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83817-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83817-1-RR against Human GABARAPL1 were performed. The affinity constant is 12.1 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maushirngewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83817-1-RR bindet in WB, IHC, IF/ICC, ELISA GABARAPL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GABARAPL1 fusion protein Ag1473
• Vollständiger Name: GABA(A) receptor-associated protein like 1
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC009309
• Gene symbol: GABARAPL1
• Gene ID (NCBI): 23710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.